Synthesis of Human Antibodies Against HBsAg in Newly Established Chinese Hamster Lung (CHL-YN) Cell Line
نویسندگان
چکیده
Hepatitis B immunoglobulin (HBIG) is an effective treatment for hepatitis B, including postexposure prophylaxis of HBV infection, prevention reinfection in liver transplant patients, and reducing sexual transmission. This study investigated the synthesis human IgG antibodies that specifically target HBsAg subtype adr CHL-YN cells, a newly established cell line grows faster than CHO-K1. To achieve antibodies, plasmid vector encoding DNA sequences against was constructed then transiently transfected into cells. The expression antigen-binding capacity recombinant were analyzed using western blot ELISA. results showed successful secretion recognize ELISA test confirmed specificity towards adr. Thus, this concluded expressed
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ژورنال
عنوان ژورنال: Journal of Mathematical and Fundamental Sciences
سال: 2023
ISSN: ['2337-5760', '2338-5510']
DOI: https://doi.org/10.5614/j.math.fund.sci.2022.54.3.1